Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V014964 | pSoup-P19 | In stock, 1 week for quality controls |
Buy one, get one free! |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
- Vector Name:
- pSoup-P19
- Antibiotic Resistance:
- Tetracycline
- Length:
- 10771 bp
- Type:
- Packaging assistance
- Replication origin:
- oriV
- Host:
- Plants
- Promoter:
- CaMV 35S
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pSoup-P19 vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
pSoup-P19 vector Sequence
LOCUS V014964 10771 bp DNA circular SYN 01-JAN-1980
DEFINITION Exported.
ACCESSION V014964
VERSION V014964
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 10771)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..10771
/mol_type="other DNA"
/organism="synthetic DNA construct"
oriT 618..727
/label="oriT"
/note="incP origin of transfer"
CDS 760..1128
/label="traJ"
/note="oriT-recognizing protein"
rep_origin 1258..1969
/label="oriV"
/note="incP origin of replication"
misc_feature 2469..2491
/label="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA
(truncated)"
promoter 2682..2700
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal complement(2776..2952)
/label="CaMV poly(A) signal"
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(3025..3540)
/note="RNA silencing suppressor p19 from Tomato bushy stunt
virus (strain Cherry). Accession#: P11690"
/label="RNA silencing suppressor p19"
promoter complement(3579..3924)
/label="CaMV 35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
promoter complement(3998..4016)
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
promoter complement(4085..4115)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(4130..4151)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4398..4426
/label="tet promoter"
/note="E. coli promoter for tetracycline efflux protein
gene"
CDS 4474..5661
/label="TcR"
/note="tetracycline efflux protein"
CDS 6163..7308
/label="trfA"
/note="trans-acting replication protein that binds to and
activates oriV"
CDS 8692..9660
/label="RepA"
/note="replication protein of plasmid pSa"
CDS 10058..10705
/label="TetR"
/note="tetracycline resistance regulatory protein"