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pEGFP-PABPN1 vector (V014751)

Basic Vector Information

Vector Name:
pEGFP-PABPN1
Antibiotic Resistance:
Kanamycin
Length:
5650 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells
Selection Marker:
Neo/G418
Promoter:
CMV
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pEGFP-PABPN1 vector Vector Map

pEGFP-PABPN15650 bp60012001800240030003600420048005400CMV enhancerCMV promoterPolyadenylate-binding protein 2EGFPSV40 poly(A) signalf1 oriAmpR promoterSV40 promoterNeoR/KanRHSV TK poly(A) signalori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pEGFP-PABPN1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V014751                 5650 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014751
VERSION     V014751
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             657..1574
                     /gene="PABPN1"
                     /label="Polyadenylate-binding protein 2"
                     /note="Polyadenylate-binding protein 2 from Homo sapiens.
                     Accession#: Q86U42"
     CDS             1596..2312
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    2438..2559
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2566..3021)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3048..3152
                     /label="AmpR promoter"
     promoter        3154..3511
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3546..4337
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4572..4619
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4948..5536
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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