Basic Vector Information
- Vector Name:
- pEF1a-UB-3×Myc-Neo
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4958 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- EF-1α
- Growth Temperature:
- 37℃
pEF1a-UB-3×Myc-Neo vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pEF1a-UB-3×Myc-Neo vector Sequence
LOCUS 62056_8900 4958 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4958) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..4958 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 123..1301 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" CDS 1328..1555 /codon_start=1 /label=ubiquitin /note="human ubiquitin C" /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG" CDS 1562..1651 /codon_start=1 /label=3xMyc /note="3 tandem Myc epitope tags" /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" polyA_signal 1799..1920 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(1927..2382) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2409..2513 /label=AmpR promoter promoter 2515..2872 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 2907..3698 /codon_start=1 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3933..3980 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4309..4897 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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