pKSS vector (V005182)

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LOCUS       V005182                 4133 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005182
VERSION     V005182
KEYWORDS    direct-selection cloning vehicle; positive-selection; pheS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Selzer G, Som T, Itoh T, Tomizawa J.
  TITLE     The origin of replication of plasmid p15A and comparative studies on
            the nucleotide sequences around the origin of related plasmids
  JOURNAL   Cell 32 (1), 119-129 (1983)
   PUBMED   6186390
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Fayat G, Mayaux JF, Sacerdot C, Fromant M, Springer M,
            Grunberg-Manago M, Blanquet S.
  TITLE     Escherichia coli phenylalanyl-tRNA synthetase operon region.
            Evidence for an attenuation mechanism. Identification of the gene
            for the ribosomal protein L20
  JOURNAL   J. Mol. Biol. 171 (3), 239-261 (1983)
   PUBMED   6317865
REFERENCE   3  (bases 3864 to 3956)
  AUTHORS   Yanisch-Perron C, Vieira J, Messing J.
  TITLE     Improved M13 phage cloning vectors and host strains: nucleotide
            sequences of the M13mp18 and pUC19 vectors
  JOURNAL   Gene 33 (1), 103-119 (1985)
   PUBMED   2985470
REFERENCE   4  (bases 1 to 4133)
  AUTHORS   Balbas P, Soberon X, Merino E, Zurita M, Lomeli H, Valle F, Flores
            N, Bolivar F.
  TITLE     Plasmid vector pBR322 and its special-purpose derivatives--a review
  JOURNAL   Gene 50 (1-3), 3-40 (1986)
   PUBMED   3034735
REFERENCE   5  (bases 4133 to 4133)
  AUTHORS   Short JM, Fernandez JM, Sorge JA, Huse WD.
  TITLE     Lambda ZAP: a bacteriophage lambda expression vector with in vivo
            excision properties
  JOURNAL   Nucleic Acids Res. 16 (15), 7583-7600 (1988)
   PUBMED   2970625
REFERENCE   6  (bases 1 to 4133)
  AUTHORS   Kast P, Hennecke H.
  TITLE     Amino acid substrate specificity of Escherichia coli
            phenylalanyl-tRNA synthetase altered by distinct mutations
  JOURNAL   J. Mol. Biol. 222 (1), 99-124 (1991)
   PUBMED   1942071
REFERENCE   7  (bases 1 to 4133)
  AUTHORS   Alting-Mees MA, Sorge JA, Short JM.
  TITLE     pBluescriptII: multifunctional cloning and mapping vectors
  JOURNAL   Meth. Enzymol. 216, 483-495 (1992)
   PUBMED   1362236
REFERENCE   8  (bases 1 to 4133)
  AUTHORS   Lin-Chao S, Chen WT, Wong TT.
  TITLE     High copy number of the pUC plasmid results from a
            Rom/Rop-suppressible point mutation in RNA II
  JOURNAL   Mol. Microbiol. 6 (22), 3385-3393 (1992)
   PUBMED   1283002
REFERENCE   9  (bases 4133 to 4133)
  AUTHORS   Kast P.
  TITLE     pKSS--a second-generation general purpose cloning vector for
            efficient positive selection of recombinant clones
  JOURNAL   Gene 138 (1-2), 109-114 (1994)
   PUBMED   8125286
REFERENCE   10  (bases 1 to 4133)
  AUTHORS   Kast P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-1993) Dept. of Chemistry and Molecular Biology,
            The Scripps Research Institute, 10550 North Torrey Pines Road, La
            Jolla, CA 92037, USA
REFERENCE   11  (bases 1 to 4133)
  TITLE     Direct Submission
REFERENCE   12  (bases 1 to 4133)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell";
            date: "1983"; volume: "32"; issue: "1"; pages: "119-129"
            SGRef: number: 2; type: "Journal Article"; journalName: "J. Mol.
            Biol."; date: "1983"; volume: "171"; issue: "3"; pages: "239-261"
            SGRef: number: 3; type: "Journal Article"; journalName: "Gene";
            date: "1985"; volume: "33"; issue: "1"; pages: "103-119"
            SGRef: number: 4; type: "Journal Article"; journalName: "Gene 50
            (1-3), 3-40 (1986)"
            SGRef: number: 5; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "1988"; volume: "16"; issue: "15"; pages:
            "7583-7600"
            SGRef: number: 6; type: "Journal Article"; journalName: "J. Mol.
            Biol."; date: "1991"; volume: "222"; issue: "1"; pages: "99-124"
            SGRef: number: 7; type: "Journal Article"; journalName: "Meth.
            Enzymol. 216, 483-495 (1992)"
            SGRef: number: 8; type: "Journal Article"; journalName: "Mol.
            Microbiol."; date: "1992"; volume: "6"; issue: "22"; pages:
            "3385-3393"
            SGRef: number: 9; type: "Journal Article"; journalName: "Gene 138
            (1-2), 109-114 (1994)"
            SGRef: number: 10; type: "Journal Article"; journalName: "Submitted
            (10-SEP-1993) Dept. of Chemistry and Molecular Biology, The Scripps
            Research Institute, 10550 North Torrey Pines Road, La Jolla, CA
            92037, USA"
            SGRef: number: 11; type: "Journal Article"
            On Oct 6, 1993 this sequence version replaced gi:403935.
FEATURES             Location/Qualifiers
     source          1..4133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     18..34
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             146..1126
                     /gene="pheS"
                     /label="Phenylalanine--tRNA ligase alpha subunit"
                     /note="Phenylalanine--tRNA ligase alpha subunit from
                     Escherichia coli O139:H28 (strain E24377A / ETEC).
                     Accession#: A7ZMI2"
     primer_bind     complement(1244..1260)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(1293..1311)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1318..1334)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      1476..1931
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1957..2061
                     /label="AmpR promoter"
     CDS             2062..2919
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3093..3681
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             3864..3956
                     /codon_start=1
                     /gene="'lacI"
                     /product="lac repressor fragment"
                     /label="'lacI"
                     /note="vestigal C-terminal portion of lac repressor; this
                     3' portion of lacI is not functional"
                     /citation=[3]
                     /citation=[7]
                     /protein_id="AAB61949.1"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     gene            3864..3956
                     /gene="'lacI"
                     /label="'lacI"
     protein_bind    3969..3990
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4005..4035
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4043..4059
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4067..4083
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4104..4122
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     old_sequence    4133
                     /replace="tg"
                     /note="all pBluescript I KS (+/-) vectors (and pKSS)
                     exhibit the deletion of the G nucleotide 5' to the KpnI
                     site [8,9]"
                     /citation=[5]
                     /citation=[7]
                     /citation=[9]