pKS336 vector (V005187)

Download GeneBank File(.gb)

LOCUS       V005187                 5368 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005187
VERSION     V005187
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Saijo M, Qing T, Niikura M, Maeda A, Ikegami T, Sakai K, Prehaud C,
            Kurane I, Morikawa S.
  TITLE     Immunofluorescence technique using HeLa cells expressing recombinant
            nucleoprotein for detection of immunoglobulin G antibodies to
            Crimean-Congo hemorrhagic fever virus
  JOURNAL   J. Clin. Microbiol. 40 (2), 372-375 (2002)
   PUBMED   11825944
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Sakai K.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUL-2001) AIDS Research Center, National Institute of
            Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011,
            Japan
REFERENCE   3  (bases 1 to 5368)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5368)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Clin.
            Microbiol."; date: "2002"; volume: "40"; issue: "2"; pages:
            "372-375"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-JUL-2001) AIDS Research Center, National Institute of Infectious
            Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..1192
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     misc_feature    1194..1243
                     /label="multiple cloning sites"
                     /note="multiple cloning sites"
     polyA_signal    complement(1239..1373)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(1798..1818)
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1948..2013)
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(2262..2681)
                     /gene="bsr"
                     /label="Blasticidin-S deaminase"
                     /note="Blasticidin-S deaminase from Bacillus cereus.
                     Accession#: P33967"
     promoter        complement(2729..3058)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     misc_feature    3209..3349
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3535..4123)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4297..5154)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5155..5259)
                     /label="AmpR promoter"