pKR43 vector (V005193)

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LOCUS       V005193                 6067 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005193
VERSION     V005193
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Rossger K, Charpin-El Hamri G, Fussenegger M.
  TITLE     Reward-based hypertension control by a synthetic brain-dopamine
            interface
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 110 (45), 18150-18155 (2013)
   PUBMED   24127594
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Roessger K, Fussenegger M, Charpin-El-Hamri G.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-AUG-2013) BSSE, ETH Zurich, Mattenstrasse 26, Basel,
            BS 4058, Switzerland
REFERENCE   3  (bases 1 to 6067)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6067)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2013"; volume: "110"; issue: "45"; pages:
            "18150-18155"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-AUG-2013) BSSE, ETH Zurich, Mattenstrasse 26, Basel, BS 4058,
            Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             656..1993
                     /gene="DRD1"
                     /label="D(1A) dopamine receptor"
                     /note="D(1A) dopamine receptor from Homo sapiens.
                     Accession#: P21728"
     CDS             2013..2729
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    2855..2976
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2983..3438)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3465..3569
                     /label="AmpR promoter"
     promoter        3571..3928
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3963..4754
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4989..5036
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5365..5953
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"