pKOS6b vector (V005198)

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LOCUS       V005198                 6689 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005198
VERSION     V005198
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Kostner D, Peters B, Mientus M, Liebl W, Ehrenreich A.
  TITLE     Importance of codB for new codA-based markerless gene deletion in
            Gluconobacter strains
  JOURNAL   Appl. Microbiol. Biotechnol. 97 (18), 8341-8349 (2013)
   PUBMED   23955475
REFERENCE   2  (bases 1 to 6689)
  AUTHORS   Kostner D, Liebl W, Ehrenreich A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2012) Department of Microbiology, TU Munich, Emil
            Ramannstr. 4, Freising, Bayern 85354, Germany
REFERENCE   3  (bases 1 to 6689)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6689)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Microbiol. Biotechnol."; date: "2013"; volume: "97"; issue: "18";
            pages: "8341-8349"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-SEP-2012) Department of Microbiology, TU Munich, Emil Ramannstr.
            4, Freising, Bayern 85354, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             401..1192
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             1495..2751
                     /gene="codB"
                     /label="Cytosine permease"
                     /note="Cytosine permease from Escherichia coli (strain
                     K12). Accession#: P0AA82"
     CDS             2744..4024
                     /label="codA"
                     /note="E. coli cytosine deaminase"
     oriT            complement(4432..4541)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     promoter        complement(4776..4866)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     promoter        complement(5060..5150)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      5510..6098
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    6386..6407
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6422..6452
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6460..6476
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6484..6500
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    6509..6565
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(6569..6585)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"