pKM24KH vector (V005207)

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LOCUS       40924_26899       12945 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pKM24KH, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12945)
  AUTHORS   Maiti IB, Sahoo DK, Raha S, Pattanaik S.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   2  (bases 1 to 12945)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 12945)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (25-MAR-2010) KTRDC Plant Genetic Engineering Research "
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(2..255)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(883..1701)
                     /label=KanR
                     /note="fusion between an N-terminal peptide of nopaline
                     synthase and Tn5 aminoglycoside phosphotransferase"
     promoter        complement(1702..1885)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    2041..2065
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      2545..3254
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(4251..4898)
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
     CDS             5004..6200
                     /label=TcR
                     /note="tetracycline efflux protein"
     CDS             complement(7479..8624)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     CDS             complement(8899..9267)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(9300..9409)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     misc_feature    9964..9988
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     repeat_region   10041..10675
                     /label=left T-DNA border repeat
                     /note="left T-DNA border repeat"
     regulatory      10041..10675
                     /label=M24 promoter from Mirabilis Mosaic Virus (MMV)
                     /note="M24 promoter from Mirabilis Mosaic Virus (MMV)"
                     /regulatory_class="promoter"
     misc_feature    10676..10720
                     /label=polylinker
                     /note="polylinker"
     terminator      10720..11361
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     promoter        11450..11629
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             11657..12676
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     terminator      12688..12937
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"