Basic Vector Information
- Vector Name:
- pKK233-2
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4593 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Amann E, Brosius J.
- Promoter:
- trc
pKK233-2 vector Vector Map
pKK233-2 vector Sequence
LOCUS 40924_26814 4593 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pKK233-2, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4593) AUTHORS Amann E, Brosius J. TITLE 'ATG vectors' for regulated high-level expression of cloned genes in Escherichia coli JOURNAL Gene 40 (2-3), 183-190 (1985) PUBMED 3007288 REFERENCE 2 (bases 1 to 4593) AUTHORS Kitts PA. TITLE CLONTECH Vectors On Disc version 1.3 JOURNAL Unpublished REFERENCE 3 (bases 1 to 4593) AUTHORS Kitts PA. TITLE Direct Submission JOURNAL Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, CA 94303, USA REFERENCE 4 (bases 1 to 4593) TITLE Direct Submission REFERENCE 5 (bases 1 to 4593) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Gene 40 (2-3), 183-190 (1985)" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, CA 94303, USA" COMMENT SGRef: number: 4; type: "Journal Article" COMMENT This vector can be obtained from CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, CA 94303, USA. To place an order call (415) 424-8222 or (800) 662-2566, extension 1. International customers, please contact your local distributor. For technical information, call (415) 424- 8222 or (800) 662-2566, extension 3. This sequence was compiled by Jurgen Brosius. If you suspect there is an error in this sequence, please contact CLONTECH's Technical Service Department at (415) 424-8222 or (800) 662-2566, extension 3 or E-mail TECH@CLONTECH.COM. FEATURES Location/Qualifiers source 1..4593 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 204..233 /label=trc promoter /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" protein_bind 241..257 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." terminator 498..584 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 676..703 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" promoter 723..814 /label=AmpR promoter CDS 815..1672 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1846..2434 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature complement(2620..2760) /label=bom /note="basis of mobility region from pBR322" CDS complement(2865..3053) /codon_start=1 /label=rop /note="Rop protein, which maintains plasmids at low copy number" /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL"
This page is informational only.