pKITE103P vector (V005229)

LOCUS       V005229                 5787 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005229
VERSION     V005229
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Toda H, Koyanagi T, Enomoto T, Itoh N.
  TITLE     Characterization of two cryptic plasmids from Kocuria palustris
            IPUFS-1 and construction of novel Escherichia coli-Kocuria shuttle
            vector for biocatalysis
  JOURNAL   J. Biosci. Bioeng. 124 (3), 255-262 (2017)
   PUBMED   28495560
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Itoh N, Toda H, Koyanagi T, Enomoto T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2016) Contact:Nobuya Itoh Toyama Prefectural
            University, Biotechnology Research Center; 5180 Kurokawa, Imizu,
            Toyama 939-0398, Japan URL :http://www.pu-toyama.ac.jp/BR/itoh/
REFERENCE   3  (bases 1 to 5787)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5787)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biosci.
            Bioeng."; date: "2017"; volume: "124"; issue: "3"; pages: "255-262"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-DEC-2016) Contact:Nobuya Itoh Toyama Prefectural University,
            Biotechnology Research Center"; volume: " 5180 Kurokawa, Imizu,
            Toyama 939-0398, Japan URL :http"; pages:
            "//www.pu-toyama.ac.jp/BR/itoh"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          4175..5781
                     /plasmid="pKPAL1"
                     /strain="IPUFS-1"
                     /mol_type="other DNA"
                     /db_xref="taxon:71999"
                     /organism="Kocuria palustris"
     misc_feature    336..392
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(396..412)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      625..1080
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1362..1466
                     /label="AmpR promoter"
     CDS             1467..2324
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2498..3086
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             3314..4120
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     CDS             4307..5182
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein"
                     /label="repA"
                     /protein_id="BAX51307.1"
                     /translation="MTTAECVQRWDQMWLPLWPLASDDLHAGVYRQARGEAMHRRYIET
                     NPRALSNLLVVDIDHEDAALRTMWNRQAWWPNAVVENPSNGHAHAVWALNEPITRTEYA
                     RRKPLAFAAAVTEGLRRSVDGDTGYSGLLTKNPAHDDWDALWCNSDRLYDLDELAEHLT
                     EGGYMPPVSWKRSKRRNSVGLGRNCSIFETARTWAYREIRNHWFDSAGLHDAISAHVHE
                     LNAEFPEPLPASEARAIAASIHRWITTKSRMWADGPAVYEATFSTIQAARGRKRVERTK
                     ALLEGWQPNG"
     gene            4307..5182
                     /gene="repA"
                     /label="repA"
     CDS             5175..5483
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein"
                     /label="repB"
                     /protein_id="BAX51308.1"
                     /translation="MGDRYDRKGSSISEMSRGTGLSPATIKRWTSRSRDEWLQQKADER
                     EAIRAFHDDEGHSWPQTAKHFRLDVSTVKRRAYRAREERKQEIAEQLQPPLPFPTNS"
     gene            5175..5483
                     /gene="repB"
                     /label="repB"