pKITE102 vector (V005231)

LOCUS       V005231                 5611 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005231
VERSION     V005231
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Toda H, Koyanagi T, Enomoto T, Itoh N.
  TITLE     Characterization of two cryptic plasmids from Kocuria palustris
            IPUFS-1 and construction of novel Escherichia coli-Kocuria shuttle
            vector for biocatalysis
  JOURNAL   J. Biosci. Bioeng. 124 (3), 255-262 (2017)
   PUBMED   28495560
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Itoh N, Toda H, Koyanagi T, Enomoto T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2016) Contact:Nobuya Itoh Toyama Prefectural
            University, Biotechnology Research Center; 5180 Kurokawa, Imizu,
            Toyama 939-0398, Japan URL :http://www.pu-toyama.ac.jp/BR/itoh/
REFERENCE   3  (bases 1 to 5611)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5611)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biosci.
            Bioeng."; date: "2017"; volume: "124"; issue: "3"; pages: "255-262"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-DEC-2016) Contact:Nobuya Itoh Toyama Prefectural University,
            Biotechnology Research Center"; volume: " 5180 Kurokawa, Imizu,
            Toyama 939-0398, Japan URL :http"; pages:
            "//www.pu-toyama.ac.jp/BR/itoh"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3999..5605
                     /plasmid="pKPAL1"
                     /strain="IPUFS-1"
                     /mol_type="other DNA"
                     /db_xref="taxon:71999"
                     /organism="Kocuria palustris"
     protein_bind    39..60
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        75..105
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    113..129
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     137..153
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    163..219
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(223..239)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      452..907
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1189..1293
                     /label="AmpR promoter"
     CDS             1294..2151
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2325..2913
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             3141..3944
                     /gene="aph"
                     /label="Aminoglycoside 3'-phosphotransferase"
                     /note="Aminoglycoside 3'-phosphotransferase from
                     Streptomyces fradiae. Accession#: P00555"
     CDS             4131..5006
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein"
                     /label="repA"
                     /protein_id="BAX51299.1"
                     /translation="MTTAECVQRWDQMWLPLWPLASDDLHAGVYRQARGEAMHRRYIET
                     NPRALSNLLVVDIDHEDAALRTMWNRQAWWPNAVVENPSNGHAHAVWALNEPITRTEYA
                     RRKPLAFAAAVTEGLRRSVDGDTGYSGLLTKNPAHDDWDALWCNSDRLYDLDELAEHLT
                     EGGYMPPVSWKRSKRRNSVGLGRNCSIFETARTWAYREIRNHWFDSAGLHDAISAHVHE
                     LNAEFPEPLPASEARAIAASIHRWITTKSRMWADGPAVYEATFSTIQAARGRKRVERTK
                     ALLEGWQPNG"
     gene            4131..5006
                     /gene="repA"
                     /label="repA"
     CDS             4999..5307
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein"
                     /label="repB"
                     /protein_id="BAX51300.1"
                     /translation="MGDRYDRKGSSISEMSRGTGLSPATIKRWTSRSRDEWLQQKADER
                     EAIRAFHDDEGHSWPQTAKHFRLDVSTVKRRAYRAREERKQEIAEQLQPPLPFPTNS"
     gene            4999..5307
                     /gene="repB"
                     /label="repB"