pKDH vector (V005248)

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LOCUS       40924_26621        6675 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pKDH, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Sahoo DK, Maiti IB.
  TITLE     Complete sequence of binary plant expression vector pKDH with 
            Hygromycin selectable marker gene
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Sahoo DK, Maiti IB.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2013) Plant Genetic Engineering Research and 
REFERENCE   3  (bases 1 to 6675)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6675)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-MAY-2013) Plant Genetic Engineering Research and Services, 
            Kentucky Tobacco Research "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(66..90)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    170..228
                     /label=1st polylinker/multiple cloning site
                     /note="1st polylinker/multiple cloning site"
     misc_feature    229..287
                     /label=2nd polylinker/multiple cloning site
                     /note="2nd polylinker/multiple cloning site"
     terminator      287..928
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     promoter        1017..1196
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             1224..2243
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     terminator      2246..2495
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2497..2550
                     /label=3rd linker/multiple cloning site
                     /note="3rd linker/multiple cloning site"
     misc_feature    complement(2648..2672)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2787..3375)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     3'UTR           complement(3399..3542)
                     /label=similar to XM2327
                     /note="similar to XM2327"
     CDS             complement(3549..4406)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4407..4511)
                     /label=AmpR promoter
     rep_origin      4540..5170
                     /label=oriV
                     /note="incP origin of replication"
     regulatory      5171..5474
                     /gene="trfA"
                     /label=similar to INSD accession X00713
                     /note="similar to INSD accession X00713"
                     /regulatory_class="promoter"
     CDS             5475..6620
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     3'UTR           6624..6675
                     /gene="trfA"
                     /label=similar to INSD accession X00713
                     /note="similar to INSD accession X00713"