Basic Vector Information
- Vector Name:
- pJZ254
- Antibiotic Resistance:
- Kanamycin
- Length:
- 11801 bp
- Type:
- Binary vector
- Replication origin:
- ori
- Host:
- Plants
- Source/Author:
- Zhang J, Khan SA, Hasse C, Ruf S, Heckel DG, Bock R.
- Promoter:
- CaMV 35S (enhanced)
pJZ254 vector Vector Map
pJZ254 vector Sequence
LOCUS 40924_26441 11801 bp DNA circular SYN 18-DEC-2018
DEFINITION Binary vector pJZ254, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11801)
AUTHORS Zhang J, Khan SA, Hasse C, Ruf S, Heckel DG, Bock R.
TITLE Pest control. Full crop protection from an insect pest by expression
of long double-stranded RNAs in plastids
JOURNAL Science 347 (6225), 991-994 (2015)
PUBMED 25722411
REFERENCE 2 (bases 1 to 11801)
AUTHORS Zhang J, Khan SA, Hasse C, Ruf S, Heckel DG, Bock R.
TITLE Direct Submission
JOURNAL Submitted (09-DEC-2014) Bock, Max Planck Institute of Molecular
Plant Physiology, Am Muehlenberg1, potsdam, Brandenburg 14476,
Germany
REFERENCE 3 (bases 1 to 11801)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 11801)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Science";
date: "2015"; volume: "347"; issue: "6225"; pages: "991-994"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(09-DEC-2014) Bock, Max Planck Institute of Molecular Plant
Physiology, Am Muehlenberg1, potsdam, Brandenburg 14476, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..11801
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 21..39
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 1057..1402
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_feature 1437..1656
/label=similar to Leptinotarsa decemlineata shrub gene
/note="similar to Leptinotarsa decemlineata shrub gene"
intron 1668..1868
/gene="St-GA20 ox"
/number=1
/note="first intron from potato GA20 oxidase gene"
gene 1668..1868
/gene="St-GA20 ox"
/label=St-GA20 ox
/note="GA20 oxidase"
misc_feature complement(1875..2094)
/label=similar to Leptinotarsa decemlineata shrub gene
/note="similar to Leptinotarsa decemlineata shrub gene"
terminator 2104..2811
/label=OCS terminator
/note="octopine synthase terminator"
promoter complement(2852..2870)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(2903..2919)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 3122..3146
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 4446..5072
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
CDS 5509..6573
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
rep_origin 6642..6836
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 7180..7320
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(7506..8094)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8184..8975)
/label=KanR
/note="aminoglycoside phosphotransferase"
regulatory complement(9178..9392)
/label=CaMV 35S terminator
/note="CaMV 35S terminator"
/regulatory_class="terminator"
misc_feature 9400..9424
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal complement(9502..9676)
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(9719..10741)
/label=HygR
/note="aminoglycoside phosphotransferase from E. coli"
promoter complement(10809..11486)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
protein_bind 11677..11698
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 11713..11743
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11751..11767
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 11775..11791
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
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