Basic Vector Information
- Vector Name:
- pJZ222
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8709 bp
- Type:
- Chloroplast transformation vector
- Replication origin:
- ori
- Source/Author:
- Zhang J, Khan SA, Hasse C, Ruf S, Heckel DG, Bock R.
- Promoter:
- lac
pJZ222 vector Vector Map
pJZ222 vector Sequence
LOCUS V005275 8709 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V005275
VERSION V005275
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 8709)
AUTHORS Zhang J, Khan SA, Hasse C, Ruf S, Heckel DG, Bock R.
TITLE Pest control. Full crop protection from an insect pest by expression
of long double-stranded RNAs in plastids
JOURNAL Science 347 (6225), 991-994 (2015)
PUBMED 25722411
REFERENCE 2 (bases 1 to 8709)
AUTHORS Zhang J, Khan SA, Hasse C, Ruf S, Heckel DG, Bock R.
TITLE Direct Submission
JOURNAL Submitted (09-DEC-2014) Bock, Max Planck Institute of Molecular
Plant Physiology, Am Muehlenberg1, potsdam, Brandenburg 14476,
Germany
REFERENCE 3 (bases 1 to 8709)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8709)
AUTHORS .
TITLE Direct Submission
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
SGRef: number: 1; type: "Journal Article"; journalName: "Science";
date: "2015"; volume: "347"; issue: "6225"; pages: "991-994"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(09-DEC-2014) Bock, Max Planck Institute of Molecular Plant
Physiology, Am Muehlenberg1, potsdam, Brandenburg 14476, Germany"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8709
/mol_type="other DNA"
/organism="synthetic DNA construct"
tRNA 31..104
/gene="trn-fM"
/product="tRNA-Met"
/label="trn-fM"
gene 31..104
/gene="trn-fM"
/label="trn-fM"
CDS complement(257..556)
/gene="rps14"
/label="Small ribosomal subunit protein uS14c"
/note="Small ribosomal subunit protein uS14c from Nicotiana
sylvestris. Accession#: Q3C1I0"
misc_feature complement(679..1879)
/label="3' part of psaB"
/note="3' part of psaB"
promoter complement(1891..1909)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1919..1935)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 2077..2532
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2558..2662
/label="AmpR promoter"
CDS 2663..3520
/label="AmpR"
/note="beta-lactamase"
rep_origin 3694..4282
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 4570..4591
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4606..4636
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 4644..4660
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4668..4684
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 4705..4723
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
misc_feature 4737..4867
/label="3' part of psbZ"
/note="3' part of psbZ"
tRNA 5143..5213
/gene="trnG"
/product="tRNA-Gly"
/label="trnG"
gene 5143..5213
/gene="trnG"
/label="trnG"
primer_bind 5418..5434
/label="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
regulatory 5443..5559
/gene="Prrn"
/regulatory_class="promoter"
gene 5443..5559
/gene="Prrn"
/label="Prrn"
misc_feature 5572..5868
/note="actin; similar to Leptinotarsa decemlineata
beta-actin"
misc_feature 5869..6088
/note="shrub; similar to Leptinotarsa decemlineata shrub
gene"
intron 6096..6296
/gene="St-GA20 ox"
/number=1
/note="first intron from potato GA20 oxidase gene"
gene 6096..6296
/gene="St-GA20 ox"
/label="St-GA20 ox"
/note="GA20 oxidase"
misc_feature complement(6310..6529)
/label="similar to Leptinotarsa decemlineata shrub gene"
/note="similar to Leptinotarsa decemlineata shrub gene"
misc_feature complement(6530..6826)
/label="similar to Leptinotarsa decemlineata actin gene"
/note="similar to Leptinotarsa decemlineata actin gene"
terminator 6835..6921
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 7013..7040
/label="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
primer_bind complement(7071..7087)
/label="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
regulatory 7112..7387
/gene="PpsbA"
/label="similar to Chlamydomonas reinhardtii psbA gene"
/note="similar to Chlamydomonas reinhardtii psbA gene"
/regulatory_class="promoter"
gene 7112..7387
/gene="PpsbA"
/label="PpsbA"
CDS 7394..8182
/label="SmR"
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
3'UTR 8200..8635
/gene="rbcL"
/label="similar to Chlamydomonas reinhardtii rbcL gene"
/note="similar to Chlamydomonas reinhardtii rbcL gene"
gene 8200..8635
/gene="rbcL"
/label="rbcL"
promoter complement(8656..8674)
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
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