pJZ199 vector (V005278)

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LOCUS       V005278                 7885 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005278
VERSION     V005278
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7885)
  AUTHORS   Zhang J, Khan SA, Hasse C, Ruf S, Heckel DG, Bock R.
  TITLE     Pest control. Full crop protection from an insect pest by expression
            of long double-stranded RNAs in plastids
  JOURNAL   Science 347 (6225), 991-994 (2015)
   PUBMED   25722411
REFERENCE   2  (bases 1 to 7885)
  AUTHORS   Zhang J, Khan SA, Hasse C, Ruf S, Heckel DG, Bock R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2014) Bock, Max Planck Institute of Molecular
            Plant Physiology, Am Muehlenberg1, potsdam, Brandenburg 14476,
            Germany
REFERENCE   3  (bases 1 to 7885)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7885)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Science";
            date: "2015"; volume: "347"; issue: "6225"; pages: "991-994"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-DEC-2014) Bock, Max Planck Institute of Molecular Plant
            Physiology, Am Muehlenberg1, potsdam, Brandenburg 14476, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     tRNA            31..104
                     /gene="trn-fM"
                     /product="tRNA-Met"
                     /label="trn-fM"
     gene            31..104
                     /gene="trn-fM"
                     /label="trn-fM"
     CDS             complement(257..556)
                     /gene="rps14"
                     /label="Small ribosomal subunit protein uS14c"
                     /note="Small ribosomal subunit protein uS14c from Nicotiana
                     sylvestris. Accession#: Q3C1I0"
     misc_feature    complement(679..1879)
                     /label="3' part of psaB"
                     /note="3' part of psaB"
     promoter        complement(1891..1909)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1919..1935)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      2077..2532
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2558..2662
                     /label="AmpR promoter"
     CDS             2663..3520
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3694..4282
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4570..4591
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4606..4636
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4644..4660
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4668..4684
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4705..4723
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    4737..4867
                     /label="3' part of psbZ"
                     /note="3' part of psbZ"
     tRNA            5143..5213
                     /gene="trnG"
                     /product="tRNA-Gly"
                     /label="trnG"
     gene            5143..5213
                     /gene="trnG"
                     /label="trnG"
     primer_bind     5419..5435
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      5444..5560
                     /gene="Prrn"
                     /regulatory_class="promoter"
     gene            5444..5560
                     /gene="Prrn"
                     /label="Prrn"
     misc_feature    5573..5869
                     /note="actin; similar to Leptinotarsa decemlineata
                     beta-actin"
     misc_feature    5870..6089
                     /note="shrub; similar to Leptinotarsa decemlineata shrub
                     gene"
     regulatory      complement(6111..6227)
                     /gene="Prrn"
                     /regulatory_class="promoter"
     gene            complement(6111..6227)
                     /gene="Prrn"
                     /label="Prrn"
     primer_bind     complement(6247..6263)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      6288..6563
                     /gene="PpsbA"
                     /label="similar to Chlamydomonas reinhardtii PpsbA"
                     /note="similar to Chlamydomonas reinhardtii PpsbA"
                     /regulatory_class="promoter"
     gene            6288..6563
                     /gene="PpsbA"
                     /label="PpsbA"
     CDS             6570..7358
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     3'UTR           7376..7811
                     /gene="rbcL"
                     /label="similar to Chlamydomonas reinhardtii rbcL 3'UTR"
                     /note="similar to Chlamydomonas reinhardtii rbcL 3'UTR"
     gene            7376..7811
                     /gene="rbcL"
                     /label="rbcL"
     promoter        complement(7832..7850)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"