pJY-mini-Tn7T-gus-Sm vector (V005280)

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LOCUS       40924_26396        7438 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJY-mini-Tn7T-gus-Sm, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7438)
  AUTHORS   Yao J, Allen C.
  TITLE     VsrAD is a major regulator of diverse virulence and fitness 
            functions in the plant pathogen Ralstonia solanacearum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7438)
  AUTHORS   Yao J, Allen C.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2007) Department of Plant Pathology, University of
            Wisconsin-Madison, 1630 Linded Drive, Madison, WI 53706, USA
REFERENCE   3  (bases 1 to 7438)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7438)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-FEB-2007) Department of Plant Pathology, University of 
            Wisconsin-Madison, 1630 Linded Drive, Madison, WI 53706, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   382..580
                     /note="Tn7R; right end of Tn7"
     primer_bind     585..601
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      629..723
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      826..912
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    946..1031
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      1036..1041
                     /regulatory_class="ribosome_binding_site"
     CDS             1050..2858
                     /label=GUS
                     /note="beta-glucuronidase"
     CDS             3761..4549
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     mobile_element  complement(4955..5120)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            complement(5279..5387)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(5619..6207)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6381..7238)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7239..7343)
                     /label=AmpR promoter