pJW3 vector (V005281)

LOCUS       40924_26391        3839 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJW3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Javid B, Wei J-R.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2012) Centre for Infectious Diseases Research, 
            Tsinghua University School of Medicine, Lab 4303, Biotechnology 
            Building, Tsinghua University, Haidian District, Beijing, Beijing 
            100084, China
REFERENCE   2  (bases 1 to 3839)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3839)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (03-AUG-2012) Centre for Infectious Diseases Research, Tsinghua 
            University School of Medicine, Lab 4303, Biotechnology Building, 
            Tsinghua University, Haidian District, Beijing, Beijing 100084, 
            China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..1902
                     /label=oriM
                     /note="oriM"
     regulatory      1909..2053
                     /label=TB HSP70 promoter
                     /note="TB HSP70 promoter"
                     /regulatory_class="promoter"
     CDS             2054..2425
                     /label=BleoR
                     /note="antibiotic-binding protein"
     CDS             2426..2449
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     rep_origin      complement(2507..2863)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     primer_bind     complement(2897..2913)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3026..3832
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"