pJS31 vector (V005287)

LOCUS       40924_26351        4491 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJS31, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Shen J, Chen J, Jensen PR, Solem C.
  TITLE     A novel genetic tool for metabolic optimization of Corynebacterium 
            glutamicum: efficient and repetitive chromosomal integration of 
            synthetic promoter-driven expression libraries
  JOURNAL   Appl. Microbiol. Biotechnol. (2017) In press
  PUBMED    28361238
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Shen J, Chen J, Jensen PR, Solem C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-DEC-2016) National Food Institute, Technical 
            University of Denmark, Soltofts Plads Building 221, 056, Lyngby 
            2800, Denmark
REFERENCE   3  (bases 1 to 4491)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4491)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Microbiol. Biotechnol. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-DEC-2016) National Food Institute, Technical University of 
            Denmark, Soltofts Plads Building 221, 056, Lyngby 2800, Denmark"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(19..52)
                     /label=lox66
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      53..100
                     /label=lysA terminator from Corynebacterium glutamicum
                     /note="lysA terminator from Corynebacterium glutamicum"
                     /regulatory_class="terminator"
     gene            101..156
                     /gene="attP"
                     /label=attP
     gene            157..199
                     /gene="attB"
                     /label=attB
     misc_feature    200..414
                     /label=multiple cloning site
                     /note="multiple cloning site"
     protein_bind    complement(415..448)
                     /label=lox71
                     /note="Left element (LE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(675..1263)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1442..2860)
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     promoter        complement(2861..3306)
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             complement(3355..4146)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"