pJPVCS vector (V005291)

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LOCUS       V005291                 6771 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005291
VERSION     V005291
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Schmelling N, Axmann IM, Dienst D.
  TITLE     The photosynthetic bacteria Rhodobacter capsulatus and Synechocystis
            sp. PCC 6803 as prokaryotic platforms for biosynthesis of cyclic
            plant triterpenes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Schmelling N, Axmann IM, Dienst D.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2017) Department of Chemistry - Microbial
            Chemistry, Angstrom and Science for Life Laboratory, Lagerhyddsvagen
            1, Uppsala 75120 Uppsala, Sweden
REFERENCE   3  (bases 1 to 6771)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6771)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-OCT-2017) Department of Chemistry - Microbial Chemistry,
            Angstrom and Science for Life Laboratory, Lagerhyddsvagen 1, Uppsala
            75120 Uppsala, Sweden"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..323
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    384..405
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     CDS             complement(407..1519)
                     /codon_start=1
                     /gene="merR"
                     /product="MerR family transcriptional regulator"
                     /label="merR"
                     /note="cobalt-dependent transcriptional regulator from
                     Synechocystis sp. PCC 6803; coaR"
                     /protein_id="ATY93172.1"
                     /translation="MKTNHLTIKELTDAVGGGVTPRMVRHYHTLGLLPPVQRSEGNYRL
                     YTQQDVQRLQRVIALKQQGFQLSHIRQLLDSHSEESLDPTLMVQLQQQYQAVIQQITRL
                     RQTASALEGLLGRDQSCQITQAEALAQLKQLDVDVQEGLGKLDQLWTNLDAETTTHPEA
                     FQESLKHLLPDLSAYSEITIHLLHQLVLACGDVSLVNAVRLSQGAIASARDALKAGCPV
                     VTDVPVVAAALDQTRLAHLGCTVKTLIDDPHITGLREAEQAFWHHDHWQQRLQQIPQGC
                     VLAIGYAPSVLLTACKLIEQQHIQPALVIGMPIGFSHAPGAKRRLMTSPIPHITIQGSL
                     GGGLLAAVTLNALVETLIAKPDCHCYLTCL"
     gene            complement(407..1519)
                     /gene="merR"
                     /label="merR"
     regulatory      1520..1600
                     /note="promoter including 5' UTR of coaT from Synechocystis
                     sp. PCC 6803 (Moscow wildtype)"
                     /regulatory_class="promoter"
     CDS             1601..2317
                     /label="Venus"
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
     terminator      2329..2400
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2416..2443
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     CDS             complement(2468..3169)
                     /gene="rppA"
                     /label="Response regulator RppA"
                     /note="Response regulator RppA from Synechocystis sp.
                     (strain PCC 6803 / Kazusa). Accession#: Q55933"
     CDS             3288..4004
                     /label="mCerulean"
                     /note="enhanced monomeric variant of CFP (Rizzo et al.,
                     2004)"
     terminator      complement(4019..4062)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      4103..4130
                     /note="phage T7 early transcription terminator; T7Te
                     terminator"
                     /regulatory_class="terminator"
     terminator      4103..4130
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     misc_feature    4137..4157
                     /label="BioBrick suffix"
                     /note="universal suffix for all parts"
     promoter        complement(4603..4633)
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(4648..4669)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4960..5548)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5722..6579)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6580..6684)
                     /label="AmpR promoter"