pJLS298 vector (V005302)

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LOCUS       V005302                 4376 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005302
VERSION     V005302
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Stoudenmire JL, Essock-Burns T, Weathers EN, Solaimanpour S, Mrazek
            J, Stabb EV.
  TITLE     An iterative synthetic approach to engineer a high-performing
            PhoB-specific reporter
  JOURNAL   Appl. Environ. Microbiol. (2018) In press
   PUBMED   29752265
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Stoudenmire JL, Stabb EV.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2018) Microbiology, University of Georgia, 120
            Cedar Street, Athens, GA 30605, USA
REFERENCE   3  (bases 1 to 4376)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4376)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-JAN-2018) Microbiology, University of Georgia, 120 Cedar Street,
            Athens, GA 30605, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             62..532
                     /gene="dhfrI"
                     /label="Dihydrofolate reductase type 1"
                     /note="Dihydrofolate reductase type 1 from Escherichia
                     coli. Accession#: P00382"
     CDS             complement(581..1288)
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     primer_bind     1390..1406
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1593..2300
                     /label="GFP"
                     /note="Aequorea victoria green fluorescent protein"
     promoter        complement(2380..2398)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2414..2802)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     oriT            complement(4075..4184)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"