pJLS149 vector (V005303)

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LOCUS       V005303                 4663 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005303
VERSION     V005303
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Stoudenmire JL, Essock-Burns T, Weathers EN, Solaimanpour S, Mrazek
            J, Stabb EV.
  TITLE     An iterative synthetic approach to engineer a high-performing
            PhoB-specific reporter
  JOURNAL   Appl. Environ. Microbiol. (2018) In press
   PUBMED   29752265
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Stoudenmire JL, Stabb EV.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2018) Microbiology, University of Georgia, 120
            Cedar Street, Athens, GA 30605, USA
REFERENCE   3  (bases 1 to 4663)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-JAN-2018) Microbiology, University of Georgia, 120 Cedar Street,
            Athens, GA 30605, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             62..532
                     /gene="dhfrI"
                     /label="Dihydrofolate reductase type 1"
                     /note="Dihydrofolate reductase type 1 from Escherichia
                     coli. Accession#: P00382"
     CDS             complement(581..1288)
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     primer_bind     1390..1406
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(1488..2567)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(2568..2645)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     protein_bind    2747..2763
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    2780..2796
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(2860..2876)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(2951..2969)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2990..3378)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     oriT            complement(4362..4471)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"