pJJH727 vector (V005322)

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LOCUS       V005322                 4824 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005322
VERSION     V005322
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Gueldener U, Heinisch J, Koehler GJ, Voss D, Hegemann JH.
  TITLE     A second set of loxP marker cassettes for Cre-mediated multiple gene
            knockouts in budding yeast
  JOURNAL   Nucleic Acids Res. 30 (6), E23 (2002)
   PUBMED   11884642
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Gueldener U, Heinisch JH, Voss D, Koehler G, Hegemann JH.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2000) Institut fuer Mikrobiologie,
            Heinrich-Heine-Universitaet, Universtitaetsstr. 1, Duesseldorf
            40225, Germany
REFERENCE   3  (bases 1 to 4824)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4824)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "2002"; volume: "30"; issue: "6"; pages: "E23"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-AUG-2000) Institut fuer Mikrobiologie,
            Heinrich-Heine-Universitaet, Universtitaetsstr. 1, Duesseldorf
            40225, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    53..86
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(243..1328)
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Kluyveromyces
                     lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267
                     / NRRL Y-1140 / WM37). Accession#: P23390"
     misc_feature    2359..2412
                     /label="loxP site"
                     /note="loxP site"
     protein_bind    complement(2375..2408)
                     /label="loxP"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     promoter        complement(2462..2480)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2738..3326)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3500..4357)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4358..4462)
                     /label="AmpR promoter"
     promoter        4808..4824
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"