pJHAM004 vector (V005327)

Basic Vector Information

Vector Name:
pJHAM004
Antibiotic Resistance:
Kanamycin
Length:
11403 bp
Type:
His-3 integration vector
Replication origin:
ori
Source/Author:
Lee DW, Haag JR, Aramayo R.

pJHAM004 vector Vector Map

pJHAM00411403 bp500100015002000250030003500400045005000550060006500700075008000850090009500100001050011000NeoR/KanRoriCAP binding siteFactor Xa sitemulti-cloning sites

pJHAM004 vector Sequence

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LOCUS       40924_26131       11403 bp DNA     circular SYN 18-DEC-2018
DEFINITION  His-3 integration vector pJHAM004, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11403)
  AUTHORS   Lee DW, Haag JR, Aramayo R.
  TITLE     Construction of strains for rapid homokaryon purification after 
            integration of constructs at the histidine-3 (his-3) locus of 
            Neurospora crassa
  JOURNAL   Curr. Genet. 43 (1), 17-23 (2003)
  PUBMED    12684841
REFERENCE   2  (bases 1 to 11403)
  AUTHORS   Lee DW, Haag JR, Aramayo R.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2002) Biology, Texas A
REFERENCE   3  (bases 1 to 11403)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11403)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr. 
            Genet."; date: "2003"; volume: "43"; issue: "1"; pages: "17-23"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-DEC-2002) Biology, Texas A"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             540..1331
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      1618..2206
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2494..2515
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(7517..7528)
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     misc_feature    7793..7845
                     /label=multi-cloning sites
                     /note="multi-cloning sites"

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