pJG105 vector (V005333)

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LOCUS       V005333                 5859 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005333
VERSION     V005333
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
  TITLE     A Broadly Conserved Antiterminator Protein Controls a Regulon of
            Bacillus amyloliquefaciens Antibiotic Gene Clusters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Goodson JR, Klupt SL.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2016) Cell Biology and Molecular Genetics,
            University of Maryland, 3112 Bioscience Research Building, College
            Park, MD 20742, USA
REFERENCE   3  (bases 1 to 5859)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5859)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2016) Cell Biology and Molecular Genetics, University of
            Maryland, 3112 Bioscience Research Building, College Park, MD 20742,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    397..1389
                     /note="LoaP Region Homology; LoaP region homology for
                     integration containing dfnA sequence"
     gene            complement(397..972)
                     /pseudo=""
                     /gene="dfnA"
                     /label="dfnA"
                     /note="fragment"
     5'UTR           complement(973..1389)
                     /note="dfnA leader region; dfnA leader region fused to
                     Pconst"
     regulatory      complement(1390..1546)
                     /label="Pconst"
                     /note="Pconst"
                     /regulatory_class="promoter"
     CDS             1997..2728
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     misc_feature    2825..3594
                     /note="LoaP Region Homology; downstream loaP homology for
                     integration into chromosome"
     gene            2825..2855
                     /pseudo=""
                     /gene="loaP"
                     /label="loaP"
                     /note="3'"
     gene            complement(3199..3594)
                     /pseudo=""
                     /locus_tag="RBA_022100"
                     /label="RBA_022100"
     primer_bind     complement(3638..3654)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3662..3678)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3686..3716)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3731..3752)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4040..4628)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4802..5659)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5660..5764)
                     /label="AmpR promoter"