pJG099 vector (V005335)

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LOCUS       V005335                10626 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005335
VERSION     V005335
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
  TITLE     A Broadly Conserved Antiterminator Protein Controls a Regulon of
            Bacillus amyloliquefaciens Antibiotic Gene Clusters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10626)
  AUTHORS   Goodson JR, Klupt SL.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2016) Cell Biology and Molecular Genetics,
            University of Maryland, 3112 Bioscience Research Building, College
            Park, MD 20742, USA
REFERENCE   3  (bases 1 to 10626)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10626)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2016) Cell Biology and Molecular Genetics, University of
            Maryland, 3112 Bioscience Research Building, College Park, MD 20742,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..107
                     /label="AmpR promoter"
     CDS             108..965
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1139..1727
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1977..2756)
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     misc_feature    3865..4865
                     /pseudo=""
                     /note="amyE; amyE back; amyE homology for integration into
                     B. amyloliquefaciens"
     CDS             complement(5239..5886)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      6196..6337
                     /note="PdfnA; promoter region of dfnA from B.
                     amyloliquefaciens FZB42"
                     /regulatory_class="promoter"
     misc_feature    6345..6622
                     /note="dfnA leader region (truncated); dfnA leader region
                     missing basepairs +76-198"
     CDS             6638..7354
                     /label="mVenus"
                     /note="Venus YFP with monomerizing A206K mutation (Nagai et
                     al., 2002; Kremers et al., 2006)"
     CDS             complement(7471..8001)
                     /codon_start=1
                     /gene="loaP"
                     /product="LoaP"
                     /label="loaP"
                     /note="LoaP; LoaP NusG-like antiterminator protein"
                     /protein_id="APU90147.1"
                     /translation="MKWYALFVESGKEETVQKFLRLQFDEQALYSIIPKKKVTERKAGI
                     KYEALKKMFPGYVLFKTKMTERTFHKIKELPISCRIVNNGAYYSKERKTYFTTIKDEEI
                     LPIIRLIGEGDTVDYSKVYIENSKVTVASGPLKGMEGIIKKIDKRKRRAKICLSFMGLD
                     KMVNVGIEVLSKP"
     gene            complement(7471..8001)
                     /gene="loaP"
                     /label="loaP"
     CDS             complement(8030..8089)
                     /codon_start=1
                     /gene="mini-xylA"
                     /product="XylA fragment"
                     /label="mini-xylA"
                     /note="mini xylA ORF"
                     /protein_id="APU90148.1"
                     /translation="MAQSHSSSVNYFVSVNKVV"
     gene            complement(8030..8089)
                     /gene="mini-xylA"
                     /label="mini-xylA"
                     /note="xylA"
     regulatory      complement(8159..8238)
                     /note="PxylA; PxylA Xylose-Inducible Promoter"
                     /regulatory_class="promoter"
     CDS             8450..9502
                     /codon_start=1
                     /gene="xylR"
                     /product="XylR Xylose Repressor protein"
                     /label="xylR"
                     /note="XylR"
                     /protein_id="APU90149.1"
                     /translation="MTGLNKSTVSSQVNTLMKENLVFEIGQGQSSGGRRPVMLVFNKKA
                     GYSIGIDVGVDYISGILTDLEGTIILDQHHHLESNSPEITKDILIDMIHHFITRMPQSP
                     YGLIGIGICVPGLIDKNQKIVFTPNSNWRDIDLKSFIQEKFNVPVFIENEANAGAYGEK
                     VFGAAKNHNNIIYASISTGIGIGVIINNHLYRGVSGFSGEMGHMTIDFNGPKCSCGNRG
                     CWELYASEKALLKSLQTKEKKVSYQDIIDLAHLNDIGTLNALQNFGFYLGIGLTNILNT
                     FNPQAIILRNSIIESHPMVLNSIRSEVSSRVYPQLGNSYELLPSSLGKNAPALGMSSIV
                     IEHFLDIVKM"
     gene            8450..9502
                     /gene="xylR"
                     /label="xylR"
     misc_feature    9510..10409
                     /pseudo=""
                     /note="amyE; amyE front; amyE homology for integration into
                     B. amyloliquefaciens"