pJG019 vector (V005344)

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LOCUS       V005344                 7911 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005344
VERSION     V005344
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
  TITLE     A Broadly Conserved Antiterminator Protein Controls a Regulon of
            Bacillus amyloliquefaciens Antibiotic Gene Clusters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7911)
  AUTHORS   Goodson JR.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2016) Cell Biology and Molecular Genetics,
            University of Maryland, 3112 Bioscience Research Building, College
            Park, MD 20742, USA
REFERENCE   3  (bases 1 to 7911)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7911)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JUL-2016) Cell Biology and Molecular Genetics, University of
            Maryland, 3112 Bioscience Research Building, College Park, MD 20742,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            complement(6..1034)
                     /pseudo=""
                     /gene="amyE"
                     /label="amyE"
                     /note="amyE-back; 3' end of amyE homology"
     CDS             complement(1408..2055)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      2368..2526
                     /gene="yfp"
                     /label="Pconst"
                     /note="Pconst"
                     /regulatory_class="promoter"
     regulatory      2533..2543
                     /gene="yfp"
                     /label="yfp RBS"
                     /note="yfp RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             2548..3264
                     /label="mVenus"
                     /note="Venus YFP with monomerizing A206K mutation (Nagai et
                     al., 2002; Kremers et al., 2006)"
     regulatory      3274..3307
                     /label="YFP_terminator"
                     /note="YFP_terminator"
                     /regulatory_class="terminator"
     gene            complement(3315..3835)
                     /pseudo=""
                     /gene="amyE"
                     /label="amyE"
                     /note="amyE-front; 5' end of amyE homology"
     promoter        4055..4159
                     /label="AmpR promoter"
     CDS             4160..5017
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5191..5779
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6029..6808)
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"