pJFR5 vector (V005345)

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LOCUS       40924_26041       10567 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJFR5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10567)
  AUTHORS   Fernandez-Rodriguez J, Moser F, Voigt CA.
  TITLE     Multichromatic Bacterial Photography
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10567)
  AUTHORS   Fernandez-Rodriguez J, Moser F, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2016) Biological Engineering, MIT, 500 Tech 
            Square, Rm 140, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 10567)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10567)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAR-2016) Biological Engineering, MIT, 500 Tech Square, Rm 140, 
            Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..19
                     /label=Promoter K1F (T7 RNAP)
                     /note="Promoter K1F (T7 RNAP)"
                     /regulatory_class="promoter"
     CDS             51..1859
                     /label=GUS
                     /note="beta-glucuronidase"
     misc_feature    1902..2061
                     /label=Terminator DT16
                     /note="Terminator DT16"
     regulatory      2112..2130
                     /label=Promoter T3 (T7 RNAP)
                     /note="Promoter T3 (T7 RNAP)"
                     /regulatory_class="promoter"
     CDS             2160..3530
                     /codon_start=1
                     /product="bFMO"
                     /label=bFMO
                     /protein_id="ARG47684.1"
                     /translation="MATRIAILGAGPSGMAQLRAFQSAQEKGAEIPELVCFEKQADWGG
                     QWNYTWRTGLDENGEPVHSSMYRYLWSNGPKECLEFADYTFDEHFGKPIASYPPREVLW
                     DYIKGRVEKAGVRKYIRFNTAVRHVEFNEDSQTFTVTVQDHTTDTIYSEEFDYVVCCTG
                     HFSTPYVPEFEGFEKFGGRILHAHDFRDALEFKDKTVLLVGSSYSAEDIGSQCYKYGAK
                     KLISCYRTAPMGYKWPENWDERPNLVRVDTENAYFADGSSEKVDAIILCTGYIHHFPFL
                     NDDLRLVTNNRLWPLNLYKGVVWEDNPKFFYIGMQDQWYSFNMFDAQAWYARDVIMGRL
                     PLPSKEEMKADSMAWREKELTLVTAEEMYTYQGDYIQNLIDMTDYPSFDIPATNKTFLE
                     WKHHKKENIMTFRDHSYRSLMTGTMAPKHHTPWIDALDDSLEAYLSDKSEIPVAKEA"
     misc_feature    3549..3698
                     /label=Terminator DT5
                     /note="Terminator DT5"
     terminator      complement(3755..3802)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(3809..3841)
                     /label=ssrA tag (LVA)
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
     misc_feature    complement(3848..3865)
                     /label=similar to SuMMV cleavage site
                     /note="similar to SuMMV cleavage site"
     CDS             complement(3887..6931)
                     /label=lacZ
                     /note="beta-galactosidase"
     regulatory      complement(6972..6994)
                     /label=Promoter CGG (T7 RNAP)
                     /note="Promoter CGG (T7 RNAP)"
                     /regulatory_class="promoter"
     CDS             complement(7656..8603)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(8651..8873)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(9365..9459)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(9493..10350)
                     /label=AmpR
                     /note="beta-lactamase"