pJFR1 vector (V005349)

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LOCUS       V005349                11252 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005349
VERSION     V005349
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11252)
  AUTHORS   Fernandez-Rodriguez J, Moser F, Voigt CA.
  TITLE     Multichromatic Bacterial Photography
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11252)
  AUTHORS   Fernandez-Rodriguez J, Moser F, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2016) Biological Engineering, MIT, 500 Tech
            Square, Rm 140, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 11252)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11252)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-MAR-2016) Biological Engineering, MIT, 500 Tech Square, Rm 140,
            Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..61
                     /label="Terminator L3S2P21"
                     /note="Terminator L3S2P21"
     misc_feature    complement(87..107)
                     /label="BioBrick suffix"
                     /note="universal suffix for all parts"
     misc_feature    complement(108..236)
                     /label="similar to SynZip 17"
                     /note="similar to SynZip 17"
     misc_feature    complement(237..257)
                     /label="GGSGGGS linker"
                     /note="GGSGGGS linker"
     misc_feature    complement(258..2057)
                     /label="similar to T7 RNA Polymerase 'core'"
                     /note="similar to T7 RNA Polymerase 'core'"
     regulatory      2088..2122
                     /label="Promoter J23105"
                     /note="Promoter J23105"
                     /regulatory_class="promoter"
     misc_feature    2172..2228
                     /label="Terminator ECK120033737"
                     /note="Terminator ECK120033737"
     CDS             complement(2241..2945)
                     /codon_start=1
                     /product="ccaR"
                     /label="ccaR"
                     /protein_id="ARG47669.1"
                     /translation="MRILLVEDDLPLAETLAEALSDQLYTVDIATDASLAWDYASRLEY
                     DLVILDVMLPELDGITLCQKWRSHSYLMPILMMTARDTINDKITGLDAGADDYVVKPVD
                     LGELFARVRALLRRGCATCQPVLEWGPIRLDPSTYEVSYDNEVLSLTRKEYSILELLLR
                     NGRRVLSRSMIIDSIWKLESPPEEDTVKVHVRSLRQKLKSAGLSADAIETVHGIGYRLA
                     NLTEKSLCQGKN"
     CDS             complement(2986..5247)
                     /codon_start=1
                     /product="ccaS"
                     /label="ccaS"
                     /protein_id="ARG47670.1"
                     /translation="MGKFLIPIEFVFLAIAMTCYLWHRQNQERRRIEISIKQQTQRERF
                     INQITQHIRQSLNLETVLNTTVAEVKTLLQVDRVLIYRIWQDGTGSAITESVNANYPSI
                     LGRTFSDEVFPVEYHQAYTKGKVRAINDIDQDDIEICLADFVKQFGVKSKLVVPILQHN
                     RASSLDNESEFPYLWGLLITHQCAFTRPWQPWEVELMKQLANQVAIAIQQSELYEQLQQ
                     LNKDLENRVEKRTQQLAATNQSLRMEISERQKTEAALRHTNHTLQSLIAASPRGIFTLN
                     LADQIQIWNPTAERIFGWTETEIIAHPELLTSNILLEDYQQFKQKVLSGMVSPSLELKC
                     QKKDGSWIEIVLSAAPLLDSEENIAGLVAVVADITEQKRQAEQIRLLQSVVVNTNDAVV
                     ITEAEPIDDPGPRILYVNEAFTKITGYTAEEMLGKTPRVLQGPKTSRTELDRVRQAISQ
                     WQSVTVEVINYRKDGSEFWVEFSLVPVANKTGFYTHWIAVQRDVTERRRTEEVRLALER
                     EKELSRLKTRFFSMASHEFRTPLSTALAAAQLLENSEVAWLDPDKRSRNLHRIQNSVKN
                     MVQLLDDILIINRAEAGKLEFNPNWLDLKLLFQQFIEEIQLSVSDQYYFDFICSAQDTK
                     ALVDERLVRSILSNLLSNAIKYSPGGGQIKIALSLDSEQIIFEVTDQGIGISPEDQKQI
                     FEPFHRGKNVRNITGTGLGLMVAKKCVDLHSGSILLKSAVDQGTTVTICLKRYNHLPRA
                     "
     promoter        complement(5283..5317)
                     /label="J23119 promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119)"
     regulatory      5363..5397
                     /label="Promoter J23106"
                     /note="Promoter J23106"
                     /regulatory_class="promoter"
     CDS             5428..7662
                     /codon_start=1
                     /product="cph8 (G722V)"
                     /label="cph8"
                     /protein_id="ARG47671.1"
                     /translation="MATTVQLSDQSLRQLETLAIHTAHLIQPHGLVVVLQEPDLTISQI
                     SANCTGILGRSPEDLLGRTLGEVFDSFQIDPIQSRLTAGQISSLNPSKLWARVMGDDFV
                     IFDGVFHRNSDGLLVCELEPAYTSDNLPFLGFYHMANAALNRLRQQANLRDFYDVIVEE
                     VRRMTGFDRVMLYRFDENNHGDVIAEDKRDDMEPYLGLHYPESDIPQPARRLFIHNPIR
                     VIPDVYGVAVPLTPAVNPSTNRAVDLTESILRSAYHCHLTYLKNMGVGASLTISLIKDG
                     HLWGLIACHHQTPKVIPFELRKACEFFGRVVFSNISAQEDTETFDYRVQLAEHEAVLLD
                     KMTTAADFVEGLTNHPDRLLGLTGSQGAAICFGEKLILVGETPDEKAVQYLLQWLENRE
                     VQDVFFTSSLSQIYPDAVNFKSVASGLLAIPIARHNFLLWFRPEVLQTVNWGGDPNHAY
                     EATQEDGKIELHPRQSFDLWKEIVRLQSLPWQSVEIQSALALKKAIVNLILRQAEELHM
                     AAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEECNAIIE
                     QFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHPLSIKRAV
                     ANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVRGDSARTIS
                     GTGLGLAIVQRIVDNHNGMLELGTSERVGLSIRAWLPVPVTRAQGTTKEG"
     misc_feature    7669..7725
                     /label="Terminator L3S2P11"
                     /note="Terminator L3S2P11"
     promoter        7751..7828
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    7829..8954
                     /label="similar to YF1"
                     /note="similar to YF1"
     CDS             8968..9582
                     /gene="fixJ"
                     /label="Transcriptional regulatory protein FixJ"
                     /note="Transcriptional regulatory protein FixJ from
                     Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC
                     13528 / IAM 13628 / NBRC 14792 / USDA 110). Accession#:
                     P23221"
     misc_feature    9595..9651
                     /label="Terminator L3S2P55"
                     /note="Terminator L3S2P55"
     rep_origin      complement(9676..10264)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(10352..10446)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(10470..11126)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"