pJET1 vector (V005350)

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LOCUS       40924_26016        3128 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Positive selection cloning vector pJET1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Lubys A, Pagarauskaite K.
  TITLE     Positive selection cloning vector pJET1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Lubys A, Pagarauskaite K.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2005) R
REFERENCE   3  (bases 1 to 3128)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3128)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-DEC-2005) R"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(187..879)
                     /codon_start=1
                     /gene="eco47IR"
                     /product="restriction endonuclease Eco47I"
                     /label=eco47IR
                     /note="restriction endonuclease; in the absence of
                     protective methylation the enzyme recognizes and cleaves 
                     double-stranded DNA targets 5'-GGWCC-3'"
                     /protein_id="ABC47403.1"
                     /translation="MSKETSFVKNAEELAKQKMDAINPELSSKFKFLIKFLSQFPEACS
                     KPRSKKMQNKVGQEEHIEYLARSFHESRLPRKPTPPTTVPDEVVSIVLNISFNIQPENL
                     ERIKEEHRFSMAAENIVGDLLERYLAEKLEPSGWIWCSGTSVKAVDFIHYDEKNNEWNL
                     LQVKNRDNTENSSSSKIRDNTTIKKWFRTYSQRDATNWDNFPDEVSSKNLNEEDFRAFV
                     KNYLAKII"
     gene            complement(187..879)
                     /gene="eco47IR"
                     /label=eco47IR
     protein_bind    complement(908..924)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(932..962)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(977..998)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1309..1897)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2071..2928)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2929..3033)
                     /label=AmpR promoter