pJC84 vector (V005355)

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LOCUS       40924_25986        3775 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Allelic replacement vector pJC84, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Wehrly TD, Chong A, Virtaneva K, Sturdevant DE, Child R, Edwards JA,
            Brouwer D, Nair V, Fischer ER, Wicke L, Curda AJ, Kupko JJ III, 
            Martens C, Crane DD, Bosio CM, Porcella SF, Celli J.
  TITLE     Intracellular biology and virulence determinants of Francisella 
            tularensis revealed by transcriptional profiling inside macrophages
  JOURNAL   Cell. Microbiol. 11 (7), 1128-1150 (2009)
  PUBMED    19388904
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Wehrly TD, Chong A, Virtaneva K, Sturdevant DE, Edwards JA, Nair V, 
            Fischer ER, Wicke L, Curda AJ, Kupko JJ III, Martens C, Crane DD, 
            Bosio CM, Porcella SF, Celli J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2008) Laboratory of Intracellular Parasites, 
            RML/NIAID/DIR/NIH, 903 South 4th street, Hamilton, MT 59840, USA
REFERENCE   3  (bases 1 to 3775)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3775)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 
            Microbiol."; date: "2009"; volume: "11"; issue: "7"; pages: 
            "1128-1150"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-AUG-2008) Laboratory of Intracellular Parasites, 
            RML/NIAID/DIR/NIH, 903 South 4th street, Hamilton, MT 59840, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(80..1498)
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     CDS             complement(1522..2313)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(2389..2658)
                     /label=Francisella tularensis groEL promoter
                     /note="Francisella tularensis groEL promoter"
                     /regulatory_class="promoter"
     misc_feature    2659..2715
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(2725..2743)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3001..3589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"