pJBkanT7POL1 vector (V005365)

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LOCUS       V005365                12196 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005365
VERSION     V005365
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12196)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12196)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 12196)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12196)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    236..257
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        272..302
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    310..326
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     334..350
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(672..3320)
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     regulatory      complement(3349..3533)
                     /label="LLT"
                     /note="LLT"
                     /regulatory_class="terminator"
     regulatory      complement(3644..3725)
                     /label="fdT"
                     /note="fdT"
                     /regulatory_class="terminator"
     terminator      3674..3722
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     terminator      complement(3901..3949)
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(4599..4664)
                     /label="lambda N peptide"
                     /note="N-terminal RNA-binding domain from the lambda
                     bacteriophage antiterminator protein N (Baron-Benhamou et
                     al., 2004)"
     misc_feature    complement(4816..4886)
                     /label="nutL"
                     /note="nutL"
     protein_bind    4945..4961
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4969..5013)
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     promoter        5355..5432
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             5433..6512
                     /label="lacI"
                     /note="lac repressor"
     CDS             6688..7500
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(7828..7844)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(8455..9600)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     regulatory      complement(9709..9758)
                     /label="neo"
                     /note="neo"
                     /regulatory_class="promoter"
     rep_origin      complement(9992..10522)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
     CDS             complement(10772..11629)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(11630..11734)
                     /label="AmpR promoter"
     oriT            11911..12019
                     /label="oriT"
                     /note="incP origin of transfer"
     primer_bind     complement(12106..12122)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"