pJB036 vector (V005372)

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LOCUS       V005372                 7067 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005372
VERSION     V005372
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Adeniran A, Stainbrook SC, Bostick JW, Tyo KEJ.
  TITLE     Detection of Peptide Biomarkers by Engineered Yeast Receptors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7067)
  AUTHORS   Pertusi D, Yu J, Adeniran A, Tyo KEJ.
  TITLE     CellSort: A support vector machine algorithm for optimizing and
            simulating FACS
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7067)
  AUTHORS   Tyo KEJ., Adeniran A, Stainbrook SC, Bostick JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2016) Chemical and Biological Engineering,
            Northwestern University, 2145 Sheridan Rd, Evanston, IL 60208, USA
REFERENCE   4  (bases 1 to 7067)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7067)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (07-MAR-2016) Chemical and Biological Engineering, Northwestern
            University, 2145 Sheridan Rd, Evanston, IL 60208, USA"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(20..36)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      39..286
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     promoter        complement(305..323)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(333..349)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      494..949
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(1083..1883)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1884..2104)
                     /label="URA3 promoter"
     misc_feature    complement(2369..2872)
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        2909..3013
                     /label="AmpR promoter"
     CDS             3014..3871
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4045..4633
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4921..4942
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4957..4987
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4995..5011
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5019..5035
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5056..5074
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5099..5742
                     /label="GAP promoter"
                     /note="promoter for glyceraldehyde-3-phosphate
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     5748..5764
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    5766..5771
                     /label="Kozak"
                     /note="Kozak"
     CDS             5772..7064
                     /gene="STE2"
                     /label="Pheromone alpha factor receptor"
                     /note="Pheromone alpha factor receptor from Saccharomyces
                     cerevisiae. Accession#: P0CI39"