pJawohl8-RNAi vector (V005373)

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LOCUS       40924_25886        9019 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pJawohl8-RNAi, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Ulker B, Lipka V, Rademacher TR, Somssich IE.
  TITLE     pJawohl8-RNAi a binary vector for gene silencing in plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9019)
  AUTHORS   Ulker B, Lipka V, Rademacher TR, Somssich IE.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2001) Biochemistry, Max-Planck-Institut 
            f.Zuechtungsforschung, Carl-von-Linne Weg 10, Koeln, NRW D-50829, 
            Germany
REFERENCE   3  (bases 1 to 9019)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9019)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-AUG-2001) Biochemistry, Max-Planck-Institut 
            f.Zuechtungsforschung, Carl-von-Linne Weg 10, Koeln, NRW D-50829, 
            Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    29..153
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        178..208
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             262..918
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1263..1565
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(1609..1733)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    1762..2048
                     /note="contains intron 1 of Arabidopsis thaliana WRKY 
                     transcription factor 33"
     protein_bind    2076..2200
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2244..2546)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(2891..3547)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3601..3631)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(3656..3780)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    4097..4121
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      4233..4942
                     /label=oriV
                     /note="incP origin of replication"
     promoter        5016..5107
                     /label=AmpR promoter
     CDS             5108..5965
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6139..6727
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    7039..7063
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(7157..7409)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7432..7980)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(8023..8202)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        8346..9019
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"