pJawohl3-RNAi vector (V005374)

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LOCUS       40924_25881        5597 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pJawohl3-RNAi, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Ulker B, Lipka V, Rademacher TR, Somssich I.
  TITLE     pJawohl3-RNAi, a binary vector for gene silencing in plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Ulker B, Lipka V, Rademacher TR, Somssich I.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-2001) Biochemistry, Max-Planck-Institut f. 
            Zuechtungsforschung, Carl-von-Linne Weg 10, Koeln, NRW D-50829, 
            Germany
REFERENCE   3  (bases 1 to 5597)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5597)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-AUG-2001) Biochemistry, Max-Planck-Institut f. 
            Zuechtungsforschung, Carl-von-Linne Weg 10, Koeln, NRW D-50829, 
            Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..50
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    51..337
                     /note="Arabidopsis thaliana putative WRKY-type DNA-binding 
                     protein (transcription factor) intron presented in Genbank 
                     Accession Number AC005499"
     misc_feature    338..386
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    387..604
                     /note="Cauliflower mosaic virus 35S polyadenylation signal"
     misc_feature    675..699
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      811..1520
                     /label=oriV
                     /note="incP origin of replication"
     promoter        1594..1685
                     /label=AmpR promoter
     CDS             1686..2543
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2717..3305
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3617..3641
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(3735..3987)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4010..4558)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(4601..4780)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4924..5597
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"