pIS375 vector (V005396)

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LOCUS       40924_25756        4950 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integration vector pIS375, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Sadowski I, Su T-C., Parent J.
  TITLE     Disintegrator vectors for single copy yeast chromosomal integration
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Sadowski I, Su T-C., Parent J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2006) Biochemistry and Molecular Biology, 
            University of British Columbia, 2350 Health Sciences Mall, 
            Vancouver, BC V6T 1Z3, Canada
REFERENCE   3  (bases 1 to 4950)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4950)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-DEC-2006) Biochemistry and Molecular Biology, University of 
            British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, 
            Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..84
                     /label=polylinker cloning sequence
                     /note="polylinker cloning sequence"
     misc_feature    85..686
                     /label=3' flanking met15 YIP-in fragment
                     /note="3' flanking met15 YIP-in fragment"
     rep_origin      complement(926..1514)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1688..2545)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2546..2650)
                     /label=AmpR promoter
     promoter        2748..2963
                     /label=URA3 promoter
     CDS             2964..3764
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        4600..4946
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"