pINV-110 vector (V005413)

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LOCUS       40924_25601        4887 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pINV-110, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R.
  TITLE     A synthetic multicellular system for programmed pattern formation
  JOURNAL   Nature 434 (7037), 1130-1134 (2005)
  PUBMED    15858574
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Basu S, Gerchman Y, Weiss R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2005) Electrical Engineering, Princeton 
            University, B-312, E-Quad, Olden Street, Princeton, NJ 08544, USA
REFERENCE   3  (bases 1 to 4887)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4887)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "2005"; volume: "434"; issue: "7037"; pages: "1130-1134"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-FEB-2005) Electrical Engineering, Princeton University, B-312, 
            E-Quad, Olden Street, Princeton, NJ 08544, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(22..1101)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(1102..1179)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     protein_bind    1245..1266
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1281..1311
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1319..1335
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1367..1378
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1385..2095
                     /label=lambda repressor
                     /note="phage lambda repressor"
     RBS             2156..2167
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      2156..2164
                     /gene="ecfP"
                     /regulatory_class="ribosome_binding_site"
     CDS             2174..2890
                     /label=ECFP
                     /note="enhanced CFP"
     terminator      2917..3003
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(3173..3718)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(3832..3926)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3960..4751)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"