pINT1-HIS3NB vector (V005419)

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LOCUS       V005419                11651 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005419
VERSION     V005419
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11651)
  AUTHORS   Meijer AH, Ouwerkerk PB, Hoge JH.
  TITLE     Vectors for transcription factor cloning and target site
            identification by means of genetic selection in yeast
  JOURNAL   Yeast 14 (15), 1407-1415 (1998)
   PUBMED   9848232
REFERENCE   2  (bases 1 to 11651)
  AUTHORS   Ouwerkerk PBF., Meijer AH.
  TITLE     Yeast one-hybrid screening for DNA-protein interactions
  JOURNAL   (in) Ausubel,F.M., Brent,R., Kingston,R.E., Moore,D.D.,
            Seidman,J.G., Smith,J.A. and Struhl,K. (Eds.); CURRENT PROTOCOLS IN
            MOLECULAR BIOLOGY: Supplement 55, Unit 12, 12.12.1-12.12.22; John
REFERENCE   3  (bases 1 to 11651)
  AUTHORS   Ouwerkerk PBF., Meijer AH.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2001) Molecular Cell Biology, Institute of
            Molecular Plant Sciences, Wassenaarseweg 64, Leiden 2333 AL,
            Netherlands
REFERENCE   4  (bases 1 to 11651)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 11651)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast";
            date: "1998"; volume: "14"; issue: "15"; pages: "1407-1415"
            SGRef: number: 2; type: "Journal Article"; journalName: "(in)
            Ausubel,F.M., Brent,R., Kingston,R.E., Moore,D.D., Seidman,J.G.,
            Smith,J.A. and Struhl,K. (Eds.)"; volume: " CURRENT PROTOCOLS IN
            MOLECULAR BIOLOGY"; pages: " Supplement 55, Unit 12,
            12.12.1-12.12.22; John Wile"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (07-NOV-2001) Molecular Cell Biology, Institute of Molecular Plant
            Sciences, Wassenaarseweg 64, Leiden 2333 AL, Netherlands"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          275..1394
                     /chromosome="VII"
                     /mol_type="genomic DNA"
                     /label="PDC6 locus flanking sequence"
                     /note="PDC6 locus flanking sequence"
                     /db_xref="taxon:4932"
                     /organism="Saccharomyces cerevisiae"
     source          6205..9242
                     /chromosome="VII"
                     /mol_type="genomic DNA"
                     /label="PDC6 locus flanking sequence"
                     /note="PDC6 locus flanking sequence"
                     /db_xref="taxon:4932"
                     /organism="Saccharomyces cerevisiae"
     protein_bind    106..127
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    1405..1452
                     /note="multiple cloning site; unique sites for NotI, XbaI
                     and SpeI"
     primer_bind     1413..1429
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1552..2208
                     /label="HIS3"
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
     regulatory      2212..2851
                     /gene="HIS3"
                     /regulatory_class="terminator"
     terminator      complement(3061..3308)
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     CDS             complement(3337..3660)
                     /gene="CYC1"
                     /label="Cytochrome c isoform 1"
                     /note="Cytochrome c isoform 1 from Saccharomyces cerevisiae
                     (strain ATCC 204508 / S288c). Accession#: P00044"
     CDS             complement(3861..4673)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(4689..6175)
                     /label="Saccharomyces cerevisiae PGK1 promoter"
                     /note="Saccharomyces cerevisiae PGK1 promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(9251..9267)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9745..9849
                     /label="AmpR promoter"
     CDS             9850..10707
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      10881..11469
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"