pINDEX4 vector (V005428)

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LOCUS       40924_25511       12072 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pINDEX4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12072)
  AUTHORS   Ouwerkerk PB, de Kam RJ, Hoge JH, Meijer AH.
  TITLE     Glucocorticoid-inducible gene expression in rice
  JOURNAL   Planta 213 (3), 370-378 (2001)
  PUBMED    11506359
REFERENCE   2  (bases 1 to 12072)
  AUTHORS   Ouwerkerk PBF.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2000) Dept. of Molecular Cell Biology, Institute 
            for Molecular Plant Sciences, Wassenaarseweg 64, Leiden 2333 AL, 
            Netherlands
REFERENCE   3  (bases 1 to 12072)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12072)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Planta"; 
            date: "2001"; volume: "213"; issue: "3"; pages: "370-378"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-AUG-2000) Dept. of Molecular Cell Biology, Institute for 
            Molecular Plant Sciences, Wassenaarseweg 64, Leiden 2333 AL, 
            Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    36..74
                     /note="multiple cloning site; restriction sites 
                     HpaI-ApaI-MunI-MluI-SwaI are unique. In here a promoter of 
                     choice to drive GVG expression can be inserted."
     CDS             321..554
                     /label=VP16 AD
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et 
                     al., 1989)"
     3'UTR           1421..1717
                     /label=Pisum sativum RbcS-E9 polyA signal
                     /note="Pisum sativum RbcS-E9 polyA signal"
     terminator      1895..2147
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      2199..2269
                     /note="4UAS; tetramer of the GVG binding site"
                     /regulatory_class="promoter"
     promoter        2318..2364
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    2395..2452
                     /note="multiple cloning site; restriction sites 
                     BbrPI-SpeI-Sse8387I-XhoI-Bsp1407I-StuI are unique."
     3'UTR           2456..2924
                     /label=Pisum sativum RbcS-3A polyA signal
                     /note="Pisum sativum RbcS-3A polyA signal"
     primer_bind     complement(2963..2979)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3182..3206
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4506..5132
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             5569..6633
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6702..6896
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7240..7380
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7566..8154)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8244..9035)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    9460..9484
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(9562..9736)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     exon            complement(9787..10439)
                     /gene="hptII"
                     /number=2
     intron          complement(10474..10663)
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     exon            complement(10670..11002)
                     /gene="hptII"
                     /number=1
     promoter        complement(11081..11758)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    11949..11970
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11985..12015
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12023..12039
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12047..12063
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"