Basic Vector Information
- Vector Name:
- pINDEX1
- Antibiotic Resistance:
- Kanamycin
- Length:
- 14203 bp
- Type:
- Binary vector
- Replication origin:
- ori
- Host:
- Plants
- Source/Author:
- Ouwerkerk PB, de Kam RJ, Hoge JH, Meijer AH.
- Promoter:
- CaMV 35S (enhanced)
pINDEX1 vector Map
pINDEX1 vector Sequence
LOCUS 40924_25496 14203 bp DNA circular SYN 18-DEC-2018
DEFINITION Binary vector pINDEX1, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14203)
AUTHORS Ouwerkerk PB, de Kam RJ, Hoge JH, Meijer AH.
TITLE Glucocorticoid-inducible gene expression in rice
JOURNAL Planta 213 (3), 370-378 (2001)
PUBMED 11506359
REFERENCE 2 (bases 1 to 14203)
AUTHORS Ouwerkerk PBF.
TITLE Direct Submission
JOURNAL Submitted (11-AUG-2000) Dept. of Molecular Cell Biology, Institute
for Molecular Plant Sciences, Wassenaarseweg 64, Leiden 2333 AL,
Netherlands
REFERENCE 3 (bases 1 to 14203)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 14203)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Planta";
date: "2001"; volume: "213"; issue: "3"; pages: "370-378"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(11-AUG-2000) Dept. of Molecular Cell Biology, Institute for
Molecular Plant Sciences, Wassenaarseweg 64, Leiden 2333 AL,
Netherlands"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..14203
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory 28..2233
/note="constitutive; derived from the Oryza sativa Gos2
encoded by Genbank Accession Number X51910"
/regulatory_class="promoter"
5'UTR join(1058..1194,2158..2238)
exon 1058..1194
/number=1
intron 1195..2157
/number=1
/note="derived from Gos2"
CDS 2479..2712
/label=VP16 AD
/note="transcriptional activation domain of herpes simplex
virus protein VP16 (Triezenberg et al., 1988; Cousens et
al., 1989)"
3'UTR 3579..3875
/label=Pisum sativum RbcS-E9 polyA signal
/note="Pisum sativum RbcS-E9 polyA signal"
terminator 4053..4305
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
regulatory 4357..4427
/note="contains 4 upstream activation sites; tetramer of
the GVG binding site"
/regulatory_class="promoter"
promoter 4476..4522
/label=minimal CaMV 35S promoter
/note="minimal 35S promoter from cauliflower mosaic virus"
misc_feature 4548..4583
/note="multiple cloning site; contains unique restriction
sites XhoI, SpeI and StuI"
3'UTR 4587..5055
/label=Pisum sativum RbcS-3A polyA signal
/note="Pisum sativum RbcS-3A polyA signal"
primer_bind complement(5094..5110)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 5313..5337
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 6637..7263
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
CDS 7700..8764
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
rep_origin 8833..9027
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 9371..9511
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(9697..10285)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(10375..11166)
/label=KanR
/note="aminoglycoside phosphotransferase"
misc_feature 11591..11615
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal complement(11693..11867)
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
exon complement(11918..12610)
/gene="hptII"
/number=2
intron complement(12605..12794)
/label=cat1 intron
/note="castor bean catalase intron, modified"
exon complement(12801..13133)
/gene="hptII"
/number=1
promoter complement(13212..13889)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
protein_bind 14080..14101
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 14116..14146
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 14154..14170
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 14178..14194
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
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