Basic Vector Information
- Vector Name:
- pIgK3conalacZ
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6882 bp
- Type:
- Reporter vector
- Replication origin:
- ori
- Source/Author:
- De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.
- Promoter:
- T3
pIgK3conalacZ vector Map
pIgK3conalacZ vector Sequence
LOCUS 40924_25436 6882 bp DNA circular SYN 18-DEC-2018 DEFINITION Reporter vector pIgK3conalacZ, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6882) AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished REFERENCE 2 (bases 1 to 6882) AUTHORS De Schamphelaire W. TITLE Direct Submission JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM REFERENCE 3 (bases 1 to 6882) TITLE Direct Submission REFERENCE 4 (bases 1 to 6882) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..6882 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin complement(3..458) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 600..616 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 626..644 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 953..1005 /label=IgK3 /note="IgK3" /regulatory_class="promoter" regulatory 1009..1172 /label=CONA /note="CONA" /regulatory_class="promoter" CDS 1264..1284 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" CDS 1315..4359 /label=lacZ /note="beta-galactosidase" polyA_signal 4510..4644 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter complement(4694..4712) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(4733..4749) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(4757..4773) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4781..4811) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(4826..4847) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(5135..5723) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5897..6754) /label=AmpR /note="beta-lactamase" promoter complement(6755..6859) /label=AmpR promoter
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