pIE1-LUC vector (V005454)

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LOCUS       40924_25336        5969 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pIE1-LUC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Zieler H, Huynh CQ.
  TITLE     Intron-dependent stimulation of marker gene expression in cultured 
            insect cells
  JOURNAL   Insect Mol. Biol. 11 (1), 87-95 (2002)
  PUBMED    11841506
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   Zieler H, Huynh CQ.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2001) Medical Entomology Section, Laboratory of 
            Parasitic Diseases, National Institutes of Health, 4 Center Drive 
            MSC 0425, Bethesda, MD 20892-0425, USA
REFERENCE   3  (bases 1 to 5969)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5969)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Insect Mol.
            Biol."; date: "2002"; volume: "11"; issue: "1"; pages: "87-95"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-OCT-2001) Medical Entomology Section, Laboratory of Parasitic 
            Diseases, National Institutes of Health, 4 Center Drive MSC 0425, 
            Bethesda, MD 20892-0425, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        111..595
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     regulatory      615..1201
                     /note="Autographa californica nuclear polyhedrosis virus 
                     immediate early promoter"
                     /regulatory_class="promoter"
     CDS             1328..2977
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    3146..3251
                     /note="Heliothis virescens p63 chaperonin gene double 
                     poly-adenylation sequence"
     CDS             4168..5025
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5199..5787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"