pIC20H-RL vector (V005455)

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LOCUS       40924_25311        9499 bp DNA     circular SYN 18-DEC-2018
DEFINITION  TREX vector pIC20H-RL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Loeschcke A, Markert A, Wilhelm S, Wirtz A, Rosenau F, Jaeger KE, 
            Drepper T.
  TITLE     TREX: A Universal Tool for the Transfer and Expression of 
            Biosynthetic Pathways in Bacteria
  JOURNAL   ACS Synth Biol 2 (1), 22-33 (2013)
  PUBMED    23656323
REFERENCE   2  (bases 1 to 9499)
  AUTHORS   Loeschcke A, Markert A, Drepper T, Jaeger K-E.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2012) Institute of Molecular Enzyme Technology, 
            Heinrich-Heine-University, Stetternicher Forst, Juelich, NRW 52426, 
            Germany
REFERENCE   3  (bases 1 to 9499)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9499)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol"; date: "2013"; volume: "2"; issue: "1"; pages: "22-33"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-SEP-2012) Institute of Molecular Enzyme Technology, 
            Heinrich-Heine-University, Stetternicher Forst, Juelich, NRW 52426, 
            Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(274..292)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             326..1117
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    1178..1196
                     /note="OE; outside end of transposon Tn5"
     oriT            1247..2438
                     /label=origin of conjuational transfer
                     /note="origin of conjuational transfer"
     promoter        2537..2565
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             2613..3800
                     /label=TcR
                     /note="tetracycline efflux protein"
     misc_feature    complement(3861..3879)
                     /note="OE; outside end of transposon Tn5"
     CDS             3953..5380
                     /label=Tn5 transposase
                     /note="transposase from the bacterial Tn5 transposon
                     (Reznikoff, 1993)"
     CDS             complement(5486..6016)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(6205..6233)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             complement(6299..7015)
                     /label=EYFP
                     /note="enhanced YFP"
     promoter        7027..7045
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7046..7070
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(7103..7119)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7593..7697
                     /label=AmpR promoter
     CDS             7698..8555
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      8729..9317
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"