pHyVec3 vector (V005467)

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LOCUS       40924_25246        5112 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHyVec3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Steele RE, Wiley CA, Chan TA.
  TITLE     Vectors for expression of cloned genes in Hydra
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Steele RE, Wiley CA, Chan TA.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2004) Dept. of Biological Chemistry, University of
            California, Irvine, 240D Medical Sciences I, Irvine, CA 92697-1700, 
            USA
REFERENCE   3  (bases 1 to 5112)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5112)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-APR-2004) Dept. of Biological Chemistry, University of 
            California, Irvine, 240D Medical Sciences I, Irvine, CA 92697-1700, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      654..1557
                     /label=Hydra vulgaris actin promoter
                     /note="Hydra vulgaris actin promoter"
                     /regulatory_class="promoter"
     misc_feature    1666..1668
                     /label=Hydra vulgaris actin start codon
                     /note="Hydra vulgaris actin start codon"
     CDS             1698..2411
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
     misc_feature    2415..2910
                     /label=3' flanking sequence from Hydra vulgaris actin
                     /note="3' flanking sequence from Hydra vulgaris actin"
     regulatory      2546..2551
                     /regulatory_class="polyA_signal_sequence"
     promoter        complement(2924..2942)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2963..2979)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2987..3003)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3011..3041)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3056..3077)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3365..3953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4127..4984)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4985..5089)
                     /label=AmpR promoter