pHY320PLK vector (V005474)

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LOCUS       40924_25211        5387 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pHY320PLK DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Ishiwa H, Shibahara H.
  TITLE     New shuttle vectors for Escherichia coli and Bacillus subtilis. III.
            Nucleotide sequence analysis of tetracycline resistance gene of 
            pAMalpha1 and ori-177
  JOURNAL   Jpn. J. Genet. 60, 485-498 (1985)
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Ishiwa H, Shibahara-Sone H.
  TITLE     New shuttle vectors for Escherichia coli and Bacillus subtilis. IV. 
            The nucleotide sequence of pHY300PLK and some properties in relation
            to transformation
  JOURNAL   Jpn. J. Genet. 61, 515-528 (1986)
REFERENCE   3  (bases 1 to 5387)
  AUTHORS   Ishiwa H, Shibahara-Sone H, Shimizu-Kadota M.
  TITLE     New shuttle vectors for Escherichia coli and Bacillus subtilis. V
  JOURNAL   Unpublished
REFERENCE   4  (bases 1 to 5387)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5387)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Jpn. J. 
            Genet. 60, 485-498 (1985)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Jpn. J. 
            Genet. 61, 515-528 (1986)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..51
                     /label=polylinker
                     /note="polylinker"
     rep_origin      102..519
                     /label=BA3 minus origin
                     /note="BA3 minus origin"
     CDS             complement(619..1992)
                     /label=TcR
                     /note="tetracycline efflux protein"
     regulatory      complement(2000..2004)
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(2125..2130)
                     /regulatory_class="minus_10_signal"
     regulatory      complement(2148..2153)
                     /regulatory_class="minus_35_signal"
     CDS             complement(2202..3203)
                     /label=repB
                     /note="RepB replication protein"
     variation       3278..3279
                     /replace="tcc"
                     /citation=[2]
     regulatory      complement(3285..3291)
                     /regulatory_class="minus_10_signal"
     regulatory      complement(3308..3313)
                     /regulatory_class="minus_35_signal"
     regulatory      complement(3336..3342)
                     /regulatory_class="minus_10_signal"
     regulatory      complement(3357..3364)
                     /regulatory_class="minus_35_signal"
     rep_origin      complement(3361..3362)
                     /direction=LEFT
                     /note="replication origin in Gram-positive bacteria (nick
                     site)"
     variation       3596
                     /citation=[2]
     variation       3699
                     /replace="c"
                     /citation=[2]
     promoter        3700..3804
                     /label=AmpR promoter
     CDS             3805..4662
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4830..5374
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     variation       5378
                     /citation=[2]