pHW001 vector (V005476)

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LOCUS       V005476                 4664 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005476
VERSION     V005476
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Yang H, Yang Z.
  TITLE     A novel way overcoming blunt-ligation in the construction of
            Streptomyces conjugative vector pHW001
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Yang H, Yang Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2006) Life Science and Technology Department,
            Biochemistry, Huazhong Agricultural University, 1 Shizishan Street,
            Wuhan, Hubei Province 430070, China
REFERENCE   3  (bases 1 to 4664)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4664)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-MAR-2006) Life Science and Technology Department, Biochemistry,
            Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei
            Province 430070, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(399..455)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     oriT            610..719
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             752..1120
                     /label="traJ"
                     /note="oriT-recognizing protein"
     CDS             complement(1410..2216)
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     regulatory      complement(2267..2272)
                     /gene="tsr"
                     /label="tandem promoter tsrp1"
                     /note="tandem promoter tsrp1"
                     /regulatory_class="minus_10_signal"
     regulatory      complement(2395..2400)
                     /gene="tsr"
                     /label="tandem promoter tsrp2"
                     /note="tandem promoter tsrp2"
                     /regulatory_class="minus_10_signal"
     primer_bind     complement(2443..2459)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2467..2483)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2491..2521)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2536..2557)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2845..3433)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3607..4464)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4465..4569)
                     /label="AmpR promoter"