Basic Vector Information
- Vector Name:
- pHTSUB-105
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 5090 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R.
pHTSUB-105 vector Map
pHTSUB-105 vector Sequence
LOCUS V005479 5090 bp DNA circular SYN 18-DEC-2018 DEFINITION Exported. ACCESSION V005479 VERSION V005479 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 5090) AUTHORS Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R. TITLE A synthetic multicellular system for programmed pattern formation JOURNAL Unpublished REFERENCE 2 (bases 1 to 5090) AUTHORS Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R. TITLE Direct Submission JOURNAL Submitted (21-JAN-2005) Electrical Engineering, Princeton University, J-319, Engineering Quadrangle, Olden Street, Princeton, NJ 08544, USA REFERENCE 3 (bases 1 to 5090) TITLE Direct Submission REFERENCE 4 (bases 1 to 5090) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (21-JAN-2005) Electrical Engineering, Princeton University, J-319, Engineering Quadrangle, Olden Street, Princeton, NJ 08544, USA" SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5090 /mol_type="other DNA" /organism="synthetic DNA construct" CDS complement(64..1143) /label="lacI" /note="lac repressor" RBS 1147..1155 /label="Shine-Dalgarno sequence" /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" CDS 1371..2120 /gene="luxR" /label="Transcriptional activator protein LuxR" /note="Transcriptional activator protein LuxR from Aliivibrio fischeri. Accession#: P12746" terminator 2191..2277 /label="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" protein_bind 2374..2395 /label="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." promoter 2410..2440 /label="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 2448..2464 /label="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2472..2488 /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" RBS 2553..2564 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" CDS 2571..3284 /label="yeGFP" /note="yeast-enhanced green fluorescent protein" rep_origin complement(3443..4031) /direction=LEFT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" terminator complement(4119..4213) /label="lambda t0 terminator" /note="transcription terminator from phage lambda" CDS complement(4237..4893) /label="CmR" /note="chloramphenicol acetyltransferase" promoter complement(4894..4996) /label="cat promoter" /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase"
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