pHT3AG vector (V005482)

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LOCUS       V005482                 8299 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005482
VERSION     V005482
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Li Q, Yuan XE, Yin YP, Wang ZK.
  TITLE     Rapid determination of exogenous plasmid copy number and stability
            using relative quantitative real-time PCR in recombinant
            Brevibacillus brevis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   Li Q, Yuan XE, Yin YP, Wang ZK.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2009) Bioengineering College of Chongqing
            University, Genetic Engineering Research Center, Shazheng Street,
            Chongqing, Chongqing 400030, China
REFERENCE   3  (bases 1 to 8299)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8299)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (29-SEP-2009) Bioengineering College of Chongqing University,
            Genetic Engineering Research Center, Shazheng Street, Chongqing,
            Chongqing 400030, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     562..578
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1678..2391
                     /label="GFP"
                     /note="Aequorea victoria green fluorescent protein"
     primer_bind     complement(2419..2435)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2443..2459)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2467..2497)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2512..2533)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2821..3409)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3583..4440)
                     /label="AmpR"
                     /note="beta-lactamase"
     CDS             complement(4595..5329)
                     /gene="erm"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Streptococcus pneumoniae. Accession#: P21236"