pHT2 vector (V005483)

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LOCUS       V005483                 4924 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005483
VERSION     V005483
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Los G, Zimprich C.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2004) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
REFERENCE   2  (bases 1 to 4924)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4924)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (08-OCT-2004) Scientific Communications, Promega Corporation, 2800
            Woods Hollow Rd., Madison, WI 53711, USA"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     promoter        1034..1052
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1075..1084
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1087..1965
                     /gene="dhaA"
                     /label="Haloalkane dehalogenase"
                     /note="Haloalkane dehalogenase from Rhodococcus sp. (strain
                     TDTM0003). Accession#: P59336"
     promoter        complement(1998..2015)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    complement(2033..2154)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      2340..2795
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3127..3231
                     /label="AmpR promoter"
     CDS             3232..4089
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4263..4851
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"