pHT-gfpBteAAV vector (V005484)

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LOCUS       V005484                 7398 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005484
VERSION     V005484
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Ben Rejeb S, Lereclus D, Slamti L.
  TITLE     Analysis of abrB Expression during the Infectious Cycle of Bacillus
            thuringiensis Reveals Population Heterogeneity
  JOURNAL   Front Microbiol 8, 2471 (2017)
   PUBMED   29312181
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Ben Rejeb S, Slamti L.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2017) MICA-Institut Micalis, INRA, Domaine de
            Vilvert, Jouy-en-Josas 78350, France
REFERENCE   3  (bases 1 to 7398)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7398)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front
            Microbiol 8, 2471 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-AUG-2017) MICA-Institut Micalis, INRA, Domaine de Vilvert,
            Jouy-en-Josas 78350, France"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             49..57
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             88..801
                     /label="superfolder GFP"
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     primer_bind     complement(887..903)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(911..927)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(935..965)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(980..1001)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1289..1877)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2051..2908)
                     /label="AmpR"
                     /note="beta-lactamase"
     CDS             complement(3071..3805)
                     /gene="erm"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Streptococcus pneumoniae. Accession#: P21236"
     primer_bind     7344..7360
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"