pHSG415s vector (V005486)

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LOCUS       V005486                 6949 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005486
VERSION     V005486
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Hashimoto-Gotoh T, Franklin FC, Nordheim A, Timmis KN.
  TITLE     Specific-purpose plasmid cloning vectors. I. Low copy number,
            temperature-sensitive, mobilization-defective pSC101-derived
            containment vectors
  JOURNAL   Gene 16 (1-3), 227-235 (1981)
   PUBMED   6282694
REFERENCE   2  (bases 1 to 6949)
  AUTHORS   Hashimoto-Gotoh T.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2002) Tam Hashimoto-Gotoh, Kyoto Pref. Univ. of
            Med., RINDG, Dept. of Biochem. and Mol. Genet.;
            Kawaramachi-Hirokoji, Kamigyo-Ku, Kyoto, Kyoto 602-8566, Japan
            (E-mail:e54093@sakura.kudpc.kyoto-u.ac.jp, Tel:81-75-251-5798,
            Fax:81-75-251-5799)
REFERENCE   3  (bases 1 to 6949)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6949)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 16
            (1-3), 227-235 (1981)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-NOV-2002) Tam Hashimoto-Gotoh, Kyoto Pref. Univ. of Med., RINDG,
            Dept. of Biochem. and Mol. Genet."; volume: " Kawaramachi-Hirokoji,
            Kamigyo-Ku, Kyoto, Kyoto 602-8566, Japan
            (E-mail:e54093@sakura.kudpc.kyoto-u.ac.jp, Tel:81-75-251-5798, Fax";
            pages: "81-75-251-5799"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(217..319)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      817..1039
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             1087..2034
                     /label="Rep101(Ts)"
                     /note="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
     CDS             complement(2959..3303)
                     /gene="mbeC"
                     /label="Mobilization protein MbeC"
                     /note="Mobilization protein MbeC from Escherichia coli.
                     Accession#: P13657"
     CDS             complement(3503..4360)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4361..4465)
                     /label="AmpR promoter"
     CDS             complement(5063..5875)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(join(6509..6949,1..216))
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"